So I did some cursory research (read: some google searches) it appears that 3/8th an inch is typically what's used on boilers that use up to 200 pounds of 'pressure' (I assume 'pressure' means PSI) and since we're only doing a quarter of that at, 50 PSI 1/4th an inch is probably sufficient though 3/8th an inch will probably be safer.
We did end up focussing on efficiency so we probably want to lean into that.
[X] 1/4th inch
[X] 3/8th inch
Don't really have a reason for horizontal, they seem to fit efficiency better but that's mostly just instincts, all steam trains we see now are horizontal so it's probably doing something right?
[X] Horizontal
Don't really know about this one, reversed tube will be better for efficiency but heavier and horizontal is already the heavier option, then again the smaller boiler thickness will probably balance that out?
[] Single large straight tube
[X] Single large reversed tube